Bioseparation Technology
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Research projects BST
Arranged chronologically, most recent at the top.
CHROMATOGRAPHY
(staff: dr.ir. Marcel Ottens)
'Process Chromatography', as opposed to analytical chromatography, deals with the separation of mixtures of near-identical biomolecules on production scale. Since many biomolecules are pharmaceutical intermediates, high-resolution separation technology such as process chromatography is crucial. The group's research focus is on the systems behaviour of Simulated Moving Bed technology, in addition to that of conventional and novel chromatographic systems. Another important aspect, integration of bioconversion and chromatography, is treated in the research theme Bioprocess Integration.
Physicochemical & Thermodynamic properties of Oligosaccharides and Peptides | Sreekanth Chilamkurti |
Physicochemical and Thermodynamic Properties of Soy and Tea Polyphenols | David Mendez Sevillano
|
High-end analytics for high-throughput biopharmaceutical process development | Alex Hanke
|
High-throughput experimentation for protein crystallization process design | Kedar Deshpande |
Beckley Kungah Nfor | |
Finished projects: | |
Tangir Ahamed | |
Esteban Freydell | |
Xiaonan Li | |
BIO-separations using Surfactant Aided gel Filtration Equipment (BIOSAFE) | Danielle Horneman |
Conversion and purification of organic acids in a SMB reactor | L.K. Cabatingan |
Self-interaction chromatography aided protein crystallization | Lin Luo |
M. de Lourdes Oliveira Maia | |
Simulated moving bed purification of polyunsaturated fatty acids from algae | P.S. Tjeerdsma |
More efficient process chromatography | J. Houwing |
Purification processes of bacterial antibiotics | T.B. Jensen |
Centrifugal partition chromatography | M.B. Buel |
Centrifugal adsorption technology | M.A.T. Bisschops |
BIOPROCESS INTEGRATION
(staff: dr.ir. Adrie J.J. Straathof)
Many known industrial bioconversions are 'difficult' since complete conversion is prevented by thermodynamic equilibrium, by product inhibition or by biocatalyst inactivation. Selectivity often is restricted by undesired degradation of starting material or product. The integration of these 'difficult' bioconversion processes with another function such as product separation is studied to obtain a better performance than in non-integrated reaction-plus-separator systems. The number of potential combinations is large and for a rational selection of operating conditions and processes, a systematic methodology for bioprocess integration at equipment and plant level is used. New types of integrated bioconversion processes are being developed.
Corjan van den Berg | |
Process synthesis for Corynebacter fermentations Process synthesis for Corynebacter fermentations | Camilo Sixto López Garzón |
Sushil Gaykawad | |
Rosario Médici | |
Integration of fermentation and recovery of carboxylic acids | Carol Roa Engel |
Integrated recovery of C4 building blocks from fermentation broth | Cagri Efe |
Countercurrent in-situ adsorptive and extractive product recovery | Arjan Oudshoorn |
Finished projects: | |
Maria C. Cuellar Soares | |
Feasibility analysis of production of ethanol from sugar cane | Cagri Efe |
Susana Sie | |
In-situ removal of solid products during whole-cell biocatalysis | Evelyn M. Buque-Taboada |
Enzymatic synthesis and purification of semi-synthetic antibiotics in a counter-current reactor | Juliana de Souza Ferreira |
Thermodynamics of antibiotics in separation processes | Susanne Rudolph |
Development of a Liquid-Liquid Centrifugal partition chromatographic Reactor | Jeroen den Hollander |
Integration of Ion Exchange chromatography and Enzymatic reaction | Marcel Janssen |
BIOPARTICLES
(staff: M. Ottens, R.G.J.M. van der Lans)
This theme deals with Manufacturing and Purification of Functional and Structured 'Bioparticles'. Most biotechnological products are solids, when sufficiently pure. New concepts based on self-assembly of biomolecules have led to new structured and functional bioparticles such as protein micro-spheres and possibly also protein crystals. A second motive for this subprogram is related to direct solids formation in the bioreactor due to enhanced product expression levels. Control of nucleation and growth phenomena as well as the selective separation of bioparticles (crystals, DNA-complexes, viral vectors, others) from mixtures of near-identical bioparticles is a field rich in fundamental and scientific challenges. In this theme subthemes may be discerned, related to making or separating bioparticles.
making particles:
Marcel Ottens: multicomponent crystallisation, protein µ-spheres, protein crystals
separating particles:
Rob van der Lans: interfacial partitioning; differential flotation; differential centrifugation
Marijana Golubovic | |
Development of a centrifugal separator for selective separation particles from complex mixtures | David Senica |
Finished projects: | |
Recovery of biologically degradable rubber with programmable properties from fermentation broth | Pim van Hee |
Partitioning and diffusion of small biomolecules in protein crystals | Aleksandar Cvetkovic |
Martijn Hoeben | |
P. Jauregi | |
Volatile acids and salts as clean auxiliary materials in protein precipitation processes | G. Hofland |
Correlative thermodynamic models for biomolecules | M. Gude |
