Electron Tomography
High resolution 3D electron microscopy: A tool for resolving the mysteries of life
Obtaining nanometer resolution in 3D electron-tomographic image volumes of frozen hydrated biological specimen opens new avenues for studying the molecular machinery inside the cell. To limit radiation damage to the sample due to the electron bombardment during a tomographic tilt series, we are restricted to low-dose series. To achieve our goals we are developing (i) quantitative forward models for designing new acquisition and reconstruction strategies; (ii) sparsity promoting image reconstruction techniques for solving underdetermined problems; (iii) image processing algorithms capable of handling very noisy data.
In collaboration with LUMC; Supported by FOM and FEI Company. Contact Bernd Rieger / Lucas van Vliet.
