Solid-state FLIM

Solid-state FLIM: The next generation of fluorescence lifetime imaging

Fluorescence lifetime imaging (FLIM) is a microscopy tool for in vivo studies of biological cells. By measuring the decay time of fluorescent markers, we can deduce their biochemical and molecular environment. The most accessible technique for FLIM is based on homodyne modulation and demodulation of excitation and emission light in a FLIM system. We are designing, building, and testing a new system based upon a CCD with custom digital electronics that obviates the need for image intensification and fiber optics thus leading to a completely solid-state system. This will improve image quality, SNR, and produce a less-expensive, more-compact system. This will not only increase the applicability of FLIM in cell biology research but should open up new application areas such as medical diagnostics and agriculture.

In collaboration with NKI-AvL, Lambert Instrument, and DALSA BV; Supported by STW and IOP Photonic Devices. Contact Ted Young / Sjoerd Stallinga / Bernd Rieger.