Prof.dr.ir. J.J. (Sef) Heijnen

Biography

• 1988 - present
  Full professor and Group leader Cell Systems Engineering (CSE), Department of Biotechnology, Faculty of Applied Sciences, Delft University of Technology
• 1985 - 1988
  Gist Brocades, Delft, The Netherlands, Research and development, Head of Department for fermentation process development
• 1984
  PhD Bioprocestechnology, Delft University of Technology, The Netherlands, Prof. N.W.F. Kossen, ‘Biological industrial waste-water treatment minimizing biomass production and maximizing biomass concentration’
• 1978 - 1985
  Gist Brocades, Delft, The Netherlands, Research and Development, Researcher and Project leader for New biological waste water treatment processes
• 1973 – 1977
  Gist Brocades, Delft, The Netherlands, Staff Engineer at Fermentation Plants for Production process optimization
• 1973
  MSc Chemical Engineering, Eindhoven University of Technology, The Netherlands, Prof. K. Rietema, ‘Fluidized bed reactors'

Member Royal Netherlands Academy of Arts and Sciences (KNAW), 2008
Co-founder NWO-NGI Center of Excellence 'Kluyver Centre for Genomics of Industrial Fermentation', 2003

Research interests

• Metabolic engineering and systems biology applied to industrial microbial processes using Saccharomyces cerevisiae, Penicillium chrysogenum and Escherichia coli
• Metabolome measurement and ¹³C-tracer analysis in steady state and dynamic conditions
• Thermodynamic and kinetic modelling of metabolism and fermentation

Teaching activities

• BSc and MSc programmes: biotechnology, fermentation technology, scale up/down, metabolic engineering, systems biology, analysis of metabolic networks
• International Advanced Courses: microbial physiology and fermentation technology, environmental biotechnology, thermodynamics
• Two-year Post-MSc programme Designer in Bioprocess Engineering: bioprocess design
• Supervised 33 PhD’s until 2008

Key publications

• Mashego, MR, Gulik, WM van & Heijnen, JJ (2007). Metabolome dynamic responses of Saccharomyces cerevisiae to simultaneous rapid perturbations in external electron acceptor and electron donor. Fems yeast research, 7, pp. 48-66. DOI
• Kresnowati, MTAP, Winden, WA van, Almering, MJH, Pierick, A ten, Ras, C, Knijnenburg, TA, Daran-Lapujade, PAS, Pronk, JT, Heijnen, JJ & Daran, JM (2006). When transcriptome meets metabolome: fast cellular responses of yeast to sudden relief of glucose limitation. Molecular systems biology, 2(49), pp. 1-16. DOI
• Wu, L., Mashego, M.R., Van Dam, J.C., Proell, A.M., Vinke, J.L., Ras, C., Van Winden, W.A., Van Gulik, W.M., Heijnen, J.J. (2005) Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly ¹³C-labeled cell extracts as internal standards. Analytical Biochemistry, 336 (2), pp. 164-171. DOI
• Heijnen, JJ (2005). Approximative kinetic formats used in metabolic network modeling - Review. Biotechnology and bioengineering, 91(5), pp. 534-545. DOI
• Van Gulik, W.M., Heijnen, J.J.A. (1995) Metabolic network stoichiometry analysis of microbial growth and product formation. Biotechnology and Bioengineering, 48 (6), pp. 681-698. DOI

Secondary employment

• Adviseur DSM - Delft
• Adviseur Mucovacx - Leiden