Dr. L.G. (Linda) Otten

Biography

• 2009 - present
  Assistant professor, Biocatalysis and Organic Chemistry group, Department of Biotechnology, Faculty of Applied Sciences, Delft University of Technology
• 2006 - 2009
  Postdoctoral Fellow, Biocatalysis and Organic Chemistry group, Department of Biotechnology, Faculty of Applied Sciences, Delft University of Technology
• 2004 - 2006
  Postdoctoral Fellow, Hollfelder group, Biochemistry department, Cambridge University, United Kingdom
• 1999 - 2003
  PhD, Pharmaceutical Biology, University of Groningen, The Netherlands (Prof. Dr. W.J. Quax)
• 1998 - 1999
  Research assistant, Section of Fine Chemicals - Organic Chemistry and Biocatalysis, DSM Geleen, The Netherlands
• 1996
  MSc, Biotechnology, University of Groningen, The Netherlands

Research interests

• Directed evolution of biocatalysts (enantioselectivity, substrate specificity)
• Novel enzymes for biotransformations
• Semi-rational enzyme engineering

Teaching activities

• PDEng program: Molecular Genetics
• Supervised 1 PhD until 2008

Key publications

• Médici, R., de María, P.D., Otten, L.G., Straathof, A.J.J. A high-throughput screening assay for amino acid decarboxylase activity (2011) Advanced Synthesis and Catalysis, 353,　pp. 2369–2376. dx.doi.org/10.1002/adsc.201100386
• Otten, L.G., Hollmann, F., Arends, I.W.C.E. Enzyme engineering for enantioselectivity: from trial-and-error to rational design? (2010) Trends in Biotechnology, 28, pp. 46-54.
  dx.doi.org/10.1016/j.tibtech.2009.10.001
• Otten, L.G., Schaffer, M.L., Villiers, B.R.M. , Stachelhaus, T., Hollfelder, F. An optimized ATP/PP_i- exchange assay in 96-well format for screening of adenylation domains for application in combinatorial chemistry (2007) Biotechnology Journal, 2, pp. 232-240.
  dx.doi.org/10.1002/biot.200600220　
• Otten, L.G., Quax, W.J. Directed evolution: selecting today's biocatalysts (2005) Biomolecular Engineering, 22, pp. 1-9.
  dx.doi.org/10.1016/j.bioeng.2005.02.002　
• Otten, L.G., Sio, C.F., Vrielink, J., Cool, R.H., Quax, W.J. Altering the substrate specificity of cephalosporin acylase by directed evolution of the β-subunit (2002) Journal of Biological Chemistry, 277, pp. 42121-42127.
  dx.doi.org/10.1074/jbc.M208317200